Microvillar Bleb Formation in Primate Hepatocytes Irradiated with 32-MeV Protons

Eight rhesus monkeys (Macaca mulatta) were whole-body irradiated with 6000 rads of 32-MeV protons. Animals were killed at several time intervals after exposure. Tissue blocks were fixed in glutaraldehyde, postosmicated, and then embedded in Maraglas-D.E.R. 732 resin. Observation of ultrathin section...

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Bibliographic Details
Published in:Radiation research 1968-11, Vol.36 (2), p.327-332
Main Authors: Ghidoni, J. J., Thomas, H.
Format: Article
Language:English
Subjects:
Animals
Blister - etiology
Blister - pathology
Cells, Cultured
Dose-Response Relationship, Radiation
Electron microscopy
Hepatocytes
Hepatocytes - pathology
Hepatocytes - radiation effects
Irradiation
Liver
Liver - pathology
Liver - radiation effects
Luminous intensity
Macaca mulatta
Microvilli
Microvilli - pathology
Microvilli - radiation effects
Primates
Protons
Protons - adverse effects
Radiation Dosage
Sine waves
Whole-Body Irradiation
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Description
Summary:Eight rhesus monkeys (Macaca mulatta) were whole-body irradiated with 6000 rads of 32-MeV protons. Animals were killed at several time intervals after exposure. Tissue blocks were fixed in glutaraldehyde, postosmicated, and then embedded in Maraglas-D.E.R. 732 resin. Observation of ultrathin sections by electron microscopy revealed balloon-like enlargements of distal portions of hepatocytic microvilli (blebs) which were less dense than the proximal portion of microvilli. The blebs ranged widely in size; some were contained within the space of Disse, whereas many protruded into the sinusoidal lumen. In a few micrographs the sinusoidal lumen was filled with bleb-like structures. Such bleb formation suggests a possible mechanistic explanation for hepatic congestion following high levels of irradiation.
ISSN:0033-7587
1938-5404
DOI:10.2307/3572655