Heme and globin synthesis and related parameters in blood from the X-irradiated duck

• Published in: Radiation research 1969, Vol.37 (1), p.50-56
• Main Author: Klein, J.R
• Format: Article
• Language: English
• Subjects: animal science; Animals; Blood; Blood - radiation effects; Blood plasma; Blood Proteins - biosynthesis; Bone marrow; Carbon Isotopes; Ducks; Erythrocytes; Erythrocytes - metabolism; Erythropoiesis - radiation effects; Globins - biosynthesis; Glycine - metabolism; Heme - biosynthesis; Hemoglobins; Hemoglobins - biosynthesis; Iron - metabolism; Iron Isotopes; Irradiation; Porphyrins; Porphyrins - metabolism; Protoporphyrins; Radiation Effects; Reticulocytes; Time Factors; zoology
• ISSN: 0033-7587; 1938-5404
• DOI: 10.2307/3572750

Decreases in the rates of heme and globin synthesis, turnover of free protoporphyrin, transfer of plasma iron to erythrocytes, and incorporation of plasma iron into heme in duck blood in vitro and in the concentration of protoporphyrin and proportion of reticulocytes in blood were observed at 1 to 8 days after x-irradiation of the animal. The decreases in rates and porphyrin concentration apparently were a consequence of the decrease in reticulocytes-that is, of erythropoietic arrest. The various parameters were found unchanged at 5 and 30 minutes after irradiation of the animal and after direct irradiation of blood. The estimations of synthesis and porphyrin turnover were based on incorporation of$\text{glycine-}2\text{-}{}^{14}{\rm C}$, the other rates on incorporation of labeled iron.