Effect of Whole-Body and Partial-Body X-Irradiation on the Extractable Cellular Components of the Lung with Special Consideration to the Alveolar Macrophage

Female Sprague-Dawley rats were given various whole-body and partial-body x-ray exposures to determine the radioresponsiveness of extractable cellular components of the lung. Cellular suspensions were obtained from the lung by intratracheal lavage with a balanced salt solution. Exposure of the thora...

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Bibliographic Details
Published in:Radiation research 1969-09, Vol.39 (3), p.716-730
Main Authors: Moyer, Robert F., Riley, Richard F.
Format: Article
Language:English
Subjects:
Alveolar macrophages
Animals
Bacteriophages
Female
Irradiation
Lung - radiation effects
Lungs
Macrophages
Macrophages - radiation effects
Organ Size
Phagocytes
Pulmonary alveoli
Pulmonary Alveoli - radiation effects
Radiation dosage
Radiation Effects
Rats
Thorax
Tissue Extracts - radiation effects
Whole body irradiation
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Summary:Female Sprague-Dawley rats were given various whole-body and partial-body x-ray exposures to determine the radioresponsiveness of extractable cellular components of the lung. Cellular suspensions were obtained from the lung by intratracheal lavage with a balanced salt solution. Exposure of the thorax to 1000 rad and whole-body irradiation at three dose levels (500, 750, and 1000 rad) all reduced extractable alveolar macrophage numbers to approximately 50% of control values during the second week after irradiation. Recovery was indicated by the fourth week. The number of polymorphonuclear leucocytes extracted from the lung increased to approximately ten times control levels during the third post-irradiation week and subsequently decreased in an exponential manner. Small aphagocytic mononuclear cells were recovered from lungs of all control rats. Their numbers were reduced prior to the third week after irradiation. Mean macrophage diameters and the frequency of binuclear macrophages were increased following x-ray exposure and reached maximal values during the third week after irradiation. An increase in lung weight following irradiation also was demonstrated. The cellular extraction procedure has been shown to be capable of providing quantitative information on cellular dynamics within the respiratory passages of the lung.
ISSN:0033-7587
1938-5404
DOI:10.2307/3572948