In Vitro Uptake of Model Amino Acids by Rat Liver Following Whole-Body γ-Irradiation

Previous in vivo studies demonstrated a stimulated hepatic capture of α-aminoisobutyric acid (AIB) following exposure of rats to whole-body γ-irradiation. This observation has been confirmed by the present in vitro studies using liver slices for the study of amino acid uptake. AIB accumulated inside...

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Bibliographic Details
Published in:Radiation research 1971-08, Vol.47 (2), p.500-510
Main Authors: Yang, Kuo-Pao, Neuhaus, Otto W.
Format: Article
Language:English
Subjects:
Active transport
Amino acids
Bicarbonates
Cell membranes
Flasks
Incubation
Irradiation
Liver
Ratios
Sodium
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Summary:Previous in vivo studies demonstrated a stimulated hepatic capture of α-aminoisobutyric acid (AIB) following exposure of rats to whole-body γ-irradiation. This observation has been confirmed by the present in vitro studies using liver slices for the study of amino acid uptake. AIB accumulated inside the cell against a concentration gradient which required an endogenous source of energy. This transport mechanism was observed to be Na+ and K+-dependent and sensitive to ouabain. Protein synthesis was needed for the stimulated AIB transport. The radiation-induced stimulation of hepatic amino acid capture was also observed with a second model amino acid, cycloleucine. However, it still remains to be established if these observations are generally applicable to the hepatic uptake of natural amino acids.
ISSN:0033-7587
1938-5404
DOI:10.2307/3573255