The Diethyldithiocarbamate Concentration Effects and Interactions with Other Cytotoxic Agents on Chinese Hamster Cells (V79)

A metal chelator, diethyldithiocarbamate (DDC) perturbs the chromosome condensation processes in dividing cells. The length of the metaphase chromosomes in Chinese hamster cells (V79) treated with 17.2 μg/ml of DDC for 2 hr is about half of that in untreated cells. However, concentrations of 1.7 μg...

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Bibliographic Details
Published in:Radiation research 1985-06, Vol.102 (3), p.271-282
Main Authors: Lin, Peck-Sun, Quamo, Stephanie, Ho, Kwei-Chi, Baur, Kenneth
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Biological effects of radiation
Bleomycin - pharmacology
Cell Line
Cell Survival - drug effects
Cell Survival - radiation effects
Chromatin
Chromosomes
Chromosomes - drug effects
Colchicine - pharmacology
Condensation
Cricetinae
Cricetulus
Cycloheximide - pharmacology
Cytotoxicity
Ditiocarb - pharmacology
DNA - metabolism
Doxorubicin - pharmacology
Drug Synergism
Edetic Acid - pharmacology
Egtazic Acid - pharmacology
Enzymes
Fundamental and applied biological sciences. Psychology
Hot Temperature
Metaphase
Oxygen
Penicillamine - pharmacology
Radioprotection
Spleen cells
Superoxides
T lymphocytes
Thiocarbamates - pharmacology
Time Factors
Tissues, organs and organisms biophysics
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Summary:A metal chelator, diethyldithiocarbamate (DDC) perturbs the chromosome condensation processes in dividing cells. The length of the metaphase chromosomes in Chinese hamster cells (V79) treated with 17.2 μg/ml of DDC for 2 hr is about half of that in untreated cells. However, concentrations of 1.7 μg or 172 μg/ml DDC apparently do not produce this effect. DDC at 17.2 μg/ml also disrupts spindle fibers. Penicillamine, EDTA, EGTA, and diamide show no effect on chromosome condensation. Bleomycin, but not mitomycin and cisplatin, added simultaneously with DDC can prevent the DDC effect on chromosomes. The cytotoxic effect of increasing concentrations of DDC to V79 cells incubated at 37°C exhibits a similar biphasic response. This concentration biphasic toxic effect is not altered when the cells are treated with DDC in combination with radiation, heat, or other cytotoxic drugs. These observations suggest that the different effects of DDC concentrations on chromosome condensation should be considered as one important modification factor for DDC related toxicity.
ISSN:0033-7587
1938-5404
DOI:10.2307/3576702