Alterations in α-Adrenergic and Muscarinic Cholinergic Receptor Binding in Rat Brain Following Nonionizing Radiation

• Published in: Radiation research 1987-01, Vol.109 (1), p.90-99
• Main Authors: Gandhi, V. C., Ross, D. H.
• Format: Article
• Language: English
• Subjects: Animals; Applied sciences; Body temperature; Body Temperature Regulation - radiation effects; Brain - metabolism; Brain - radiation effects; Brain Mapping; Cholinergic receptors; Cholinergics; Clonidine; Dose-Response Relationship, Radiation; Exact sciences and technology; Hyperthermia; Hypothalamus; Hypothalamus - metabolism; Hypothalamus - radiation effects; Male; Microwaves; Other techniques and industries; Quinuclidinyl Benzilate; Rats; Receptors; Receptors, Adrenergic, alpha - metabolism; Receptors, Muscarinic - metabolism; Temperature control; Thermoregulation
• ISSN: 0033-7587; 1938-5404
• DOI: 10.2307/3576870

Microwave radiation produces hyperthermia. The mammalian thermoregulatory system defends against changes in temperature by mobilizing diverse control mechanisms. Neurotransmitters play a major role in eliciting thermoregulatory responses. The involvement of adrenergic and muscarinic cholinergic receptors was investigated in radiation-induced hyperthermia. Rats were subjected to radiation at 700 MHz frequency and $15\ {\rm mW}/{\rm cm}^{2}$ power density and the body temperature was raised by 2.5°C. Of six brain regions investigated only the hypothalamus showed significant changes in receptor states, confirming its pivotal role in thermoregulation. Adrenergic receptors, studied by $[{}^{3}{\rm H}]\text{clonidine}$ binding, showed a 36% decrease in binding following radiation after a 2.5°C increase in body temperature, suggesting a mechanism to facilitate norepinephrine release. Norepinephrine may be speculated to maintain thermal homeostasis by activating heat dissipation. Muscarinic cholinergic receptors, studied by [3 H]quinuclidinyl benzilate binding, showed a 65% increase in binding at the onset of radiation. This may be attributed to the release of acetylcholine in the hypothalamus in response to heat cumulation. The continued elevated binding during the period of cooling after radiation was shut off may suggest the existence of an extra-hypothalamic heat-loss pathway.