Characterization of Antibodies Produced to Recombinant Polyomavirus Structural Proteins

Four polyomavirus antisera were examined and characterized. These antisera included a prokaryotic expressed recombinant VP1 antiserum, a baculovirus expressed recombinant VP1 antiserum, a baculovirus expressed recombinant VP1/2 antiserum, and, for comparison, a virion polyclonal antiserum. The respe...

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Bibliographic Details
Published in:Transactions of the Kansas Academy of Science 1998-01, Vol.101 (3/4), p.125-131
Main Authors: Thompson, Stefanie G., Reeves, Wendy M., An, Ke, Consigli, Richard A.
Format: Article
Language:English
Subjects:
Antibodies
Antiserum
Baculoviridae
Capsid
Capsid proteins
Cellular immunity
Hemagglutination
Polyomavirus
Virions
Virology
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Summary:Four polyomavirus antisera were examined and characterized. These antisera included a prokaryotic expressed recombinant VP1 antiserum, a baculovirus expressed recombinant VP1 antiserum, a baculovirus expressed recombinant VP1/2 antiserum, and, for comparison, a virion polyclonal antiserum. The respective antisera were characterized for their ability to inhibit hemagglutination, to neutralize virus infection, and to detect polyomavirus proteins by immunofluorescence and Western blot assays. This research indicates that prokaryotic and eukaryotic expressed sources of the same antigenic proteins influence the properties of the antibodies produced.
ISSN:0022-8443
1938-5420
DOI:10.2307/3627960