Arachidonic acid released from epithelial cells by Malassezia furfur phospholipase A2: a potential pathophysiologic mechanism

Malassezia furfur is a lipophilic yeast that has been related to inflammatory skin conditions such as seborrhoeic dermatitis and psoriasis. We have previously reported that this yeast secretes enzymes capable of metabolizing complex lipids. This lipolytic system is critical for M. furfur growth, sin...

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Bibliographic Details
Published in:Mycologia 1998-03, Vol.90 (2), p.163-169
Main Authors: Plotkin, L.I. (CONICET-UBA, Buenos Aires, Argentina.), Mathov, I, Squiquera, L, Leoni, J
Format: Article
Language:English
Subjects:
ACIDE ARACHIDONIQUE
ACIDO ARAQUIDONICO
ACTIVIDAD ENZIMATICA
ACTIVITE ENZYMATIQUE
ANATOMIA ANIMAL
ANATOMIE ANIMALE
ANIMAL MORPHOLOGY
Animal Pathogens
ARACHIDONIC ACID
Cell membranes
Cellular metabolism
Chemical suspensions
Cultured cells
DERMATITE
DERMATITIS
DEVELOPMENTAL STAGES
ENFERMEDADES DE LA PIEL
ENZYMIC ACTIVITY
EPITELIO
Epithelial cells
EPITHELIUM
ESTERASAS
ESTERASE
ESTERASES
ETAPAS DE DESARROLLO
GENERO HUMANO
GENRE HUMAIN
Hemolysis
INFLAMACION
INFLAMMATION
LIPIDE
LIPIDOS
LIPIDS
MALADIE DE LA PEAU
MALASSEZIA
MANKIND
METABOLISM
METABOLISME
METABOLISMO
METABOLITE
METABOLITES
METABOLITOS
PSORIASIS
Skin
SKIN DISEASES
STADE DE DEVELOPPEMENT
Yeasts
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Description
Summary:Malassezia furfur is a lipophilic yeast that has been related to inflammatory skin conditions such as seborrhoeic dermatitis and psoriasis. We have previously reported that this yeast secretes enzymes capable of metabolizing complex lipids. This lipolytic system is critical for M. furfur growth, since the yeast has a strict dependence on an exogenous source of fatty acids. In the present study we show that M. furfur can grow in cultures supplemented with either Hep-2 cell membranes or erythrocytes as the sole source of lipids. M. furfur secretes enzymes having phospholipase A2 activity, based upon the lysis of erythrocytes being inhibited by pretreatment with p-bromophenacyl-bromide. This enzyme liberates arachidonic acid from Hep-2 cells, as confirmed by High Performance Liquid Chromatography and High Performance Thin Layer Chromatography analysis. Arachidonic acid metabolites are well-known mediators of skin inflammation. Biopsies taken after topical inoculations of mice with M. furfur culture supernatant show evidence of an inflammatory response. As a result of our findings, we established that M. furfur can trigger an inflammatory response per se by releasing metabolic products.
ISSN:0027-5514
1557-2536
DOI:10.2307/3761291