Elevation of the Soluble form GPI-80, a β2 Integrin-associated Glycosylphosphatidylinositol Anchored Protein, in the Serum of Patients with Wegener's Granulomatosis

• Published in: Allergology international 2005, Vol.54 (2), p.299-303
• Main Authors: Yoshitake, Hiroshi, Nitto, Takeaki, Ohta, Nobuo, Fukase, Shigeru, Aoyagi, Masaru, Sendo, Fujiro, Araki, Yoshihiko
• Format: Article
• Language: English
• Subjects: anti-neutrophil cytoplasmic antibodies; enzyme-Linked immunosorbent assay; neutrophils; tumor necrosis factor receptors; Wegener's granulomatosis
• ISSN: 1323-8930; 1440-1592
• DOI: 10.2332/allergolint.54.299

The purpose of present study is to determine the value of serum levels of the soluble form of β2 integrin-associated glycosylphosphatidylinositol (GPI)-anchored glycoprotein (GPI-80) for monitoring disease activity during the clinical course of the disease, in a total of 24 serum samples from 9 patients with Wegener's granulomatosis (WG). The serum soluble form GPI-80 (sGPI-80) concentrations of WG patients were measured by enzyme-linked immunosorbent assay (ELISA). The titers of anti-neutrophil cytoplasmic antibody (ANCA) were assessed by an indirect immunofluorescence method. The serum concentrations of tumor necrosis factor-a (TNF-a), soluble TNF receptor (sTNFR) I and II, soluble interleukin-2 receptor, and soluble intracellular adhesion molecule-1 of WG patients were also measured by ELISA. The serum sGPI-80 levels were significantly elevated in active WG and were positively correlated with disease activity. At the time of remission, a significant decrease in GPI-80 was observed. The serum sGPI- 80 levels were correlated with the ANCA titer and the concentrations of sTNFR I and II. These findings suggest that sGPI-80 is useful as an additional measure of WG activity.