Near-infrared laser cellular ablation and development in Xenopus laevis embryos

The biological applications of pulsed near-infrared (NIR) lasers are widening due to potential use of this technology to manipulate cellular structure. The present study was conducted to further explore the efficacy of NIR lasers for ablating individual cells in eight-cell stage Xenopus laevis embry...

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Bibliographic Details
Published in:Journal of laser applications 2016-02, Vol.28 (1)
Main Authors: Mulholland, Grace E., Moore, Pamela, Khamsi, Youssef, McMillan, Jameel, Sible, Jill C., Khodaparast, Giti A.
Format: Article
Language:English
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Summary:The biological applications of pulsed near-infrared (NIR) lasers are widening due to potential use of this technology to manipulate cellular structure. The present study was conducted to further explore the efficacy of NIR lasers for ablating individual cells in eight-cell stage Xenopus laevis embryos. Ablations were performed with four experimental groups, at magnifications of 10× and 40× (laser beam radius of 0.32 and 0.23 μm, respectively) with either one cell or two adjacent cells targeted. The survivorship, size, and phenotypic mutations of each group were documented and compared to a control group. Survivorship was not affected in any experimental group; however, statistically significant differences were noted in embryonic length (p = 0.02) and in morphology (p 
ISSN:1042-346X
1938-1387
DOI:10.2351/1.4936152