Determination of antioxidant activity using oxidative damage to plasmid DNA — pursuit of solvent optimization

• Published in: Chemical papers 2013-05, Vol.67 (5), p.484-489
• Main Authors: Treml, Jakub, Šmejkal, Karel, Hošek, Jan, Žemlička, Milan
• Format: Article
• Language: English
• Subjects: antioxidant activity; Biochemistry; Biotechnology; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; diplacone; Fenton reaction; Industrial Chemistry/Chemical Engineering; Materials Science; Medicinal Chemistry; Original Paper; plasmid DNA; solubility
• ISSN: 0366-6352; 1336-9075; 1336-9075
• DOI: 10.2478/s11696-013-0334-8

Oxidative stress plays a key role in the pathophysiology of many diseases. Hydroxyl radical is the oxidative species most commonly causing damage to cells. The aim of this work was to optimize the method for antioxidant activity determination on a model lipophilic geranylated flavanone, diplacone. This method uses protection of plasmid DNA from oxidation by a hydroxyl radical generated by the Fenton reaction involving oxidation of metal ions using H 2 O 2 and ascorbate. The method was optimized for lipophilic compounds using several solvents and co-solvents. It was found that (2-hydroxypropyl)- β -cyclodextrin (0.1 mass % aq. sol.) is the best co-solvent for our model lipophilic compound to measure the antioxidant activity by the method presented. Other solvents, namely dimethyl sulfoxide, Cremophor EL® (0.1 mass % aq. sol.), ethanol, and methanol, were not suitable for the determination of the antioxidant activity by the method described. Tween 80 (0.1 mass % aq. sol.) and a mixture of 10 vol. % ethanol and 9 mass % bovine serum albumin (aq. sol.) significantly decreased the antioxidant activity of the model lipophilic compound and thus were not suitable for this method.