Tropospheric ozone-induced structural changes in leaf mesophyll cell walls in grapevine plants

• Published in: Biológia 2006-01, Vol.61 (1), p.85-90
• Main Authors: Ljubesic, N.(Ruder Boskovic Institute, Zagreb (Croatia), Britvec, M.University of Zagreb (Croatia). Faculty of Agriculture
• Format: Article
• Language: English
• Subjects: ANALISIS DE TEJIDOS; ANALYSE DE TISSUS; callose; cell wall; CELL WALLS; FEUILLE; grapevine; GRAPEVINES; HOJAS; leaf structure; LEAVES; MESOFILO; MESOPHYLL; MESOPHYLLE; OZONE; OZONO; PARED CELULAR; PAROI CELLULAIRE; TISSUE ANALYSIS; tropospheric ozone; VID; VIGNE
• ISSN: 0006-3088; 1336-9563
• DOI: 10.2478/s11756-006-0012-1

The structural changes in leaves of grapevine plants (Vitis vinifera L.) exposed to different ozone concentrations were investigated. Ozone fumigations were performed in open-top chambers at four different ozone levels (charcoal-filtered air (F), ambient air (N), ambient air + 25 cubic mm m(sup-3) ozone (O-25) and ambient air -+- 50 cubic mm m(sup-3) ozone (O-50)). The leaves of plants from chambers with increased ozone concentrations (O-25 and O-50) were significantly thicker than the controls (F), owing to increased thickness of the mesophyll layer. Observing O-50 leaves, it was found that the mesophyll cell wall displayed structural changes. In some places cell wall thickness increased up to 1 micro. We found callose deposits on the inner side of the cell walls of mesophyll cells. These data are in accord with the concept that the mesophyll cell wall acts as a barrier against the penetration of tropospheric ozone into the cells.