Evaluation of Honeycomb Scaffold Combined with KUSA/A1 Cells for Tissue Engineering

We evaluated the efficacy of honeycomb scaffold combined with KUSA/A1 cells in vivo. The transplants were subjected to radiographical, histological and immunohistochemical examinations after 2 and 4 weeks of implantation. KUSA/A1 cells alone showed small nests of bone formation. Whereas, KUSA/A1-Ate...

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Bibliographic Details
Published in:Journal of Hard Tissue Biology 2005, Vol.14(2), pp.223-224
Main Authors: Takagi, Tohru, Rodriguez, Andrea Paola, Borkosky, Silvia Susana, Rui, Kan, Haillong, Hu, Oida, Shinichiro, Komuro, Miki, Nagatsuka, Hitoshi, Nagai, Noriyuki
Format: Article
Language:English
Subjects:
atelocollagen
bone formation
honeycomb scaffold
KUSA/A1 cells
tissue engineering
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Summary:We evaluated the efficacy of honeycomb scaffold combined with KUSA/A1 cells in vivo. The transplants were subjected to radiographical, histological and immunohistochemical examinations after 2 and 4 weeks of implantation. KUSA/A1 cells alone showed small nests of bone formation. Whereas, KUSA/A1-Atelocollagen revealed abundant new bone formation. We also determined the immunolocalization of type I collagen, CD34, Osteocalcin, and PCNA in this newly formed bone. Our results indicated that collagen scaffold plays an important role allowing vessel formation and cell anchorage especially through the proliferation and differentiation process in a confined space. This study can possible enhances existing therapeutic applications.
ISSN:1341-7649
1880-828X
DOI:10.2485/jhtb.14.223