Effect of Synthetic Red Dye Orange Red and Natural Red Dye Alizarin on Biochemical and Hematological Parameters in Male Wistar Rats

• Published in: International journal of pharmaceutical sciences and drug research 2019-09, p.221-225
• Main Authors: Khan, Ishfaq Shafi, Ali, Md Niamat, Murtaza, Mohd
• Format: Article
• Language: English
• ISSN: 0975-248X; 0975-248X
• DOI: 10.25004/IJPSDR.2019.110511

Nowadays synthetic food dyes are mostly preferred than natural plant derived dyes due to low cost and intense coloration. In this study hematological and biochemical parameters were determined in male wistar rats after 30 days treatment with synthetic red dye orange red and natural plant derived red dye alizarin. 25 male wistar rats were divided into 5 groups with 5 animals per group. Group I rats were taken as control treated with normal rat diet and distilled water. Group II and III rats (experimental) were oral gavaged with 50 mg and 150 mg/kg body weight of alizarin dye. Group IV and V rats (experimental) were gavaged with 50 mg and 150 mg/kg body weight of orange red dye. Treatment of group V rats with 150 mg/kg body weight of orange red dye produce significant changes in RBC, Hb, Hct, MCH, serum aminotransferase enzymes and serum protein fraction. In comparison to this in group IV rats a significant change was observed only in Hb, serum aminotransferase enzymes and serum protein fraction when compared with control (group I) rats. However in group II and III alizarin treated rats no significant change was observed in different biochemical and hematological parameters relative to their respective control. In conclusion synthetic orange red dye proved to be more toxic than natural plant derived red dye alizarin.