Determination of ammonia by enzyme electrode with NAD amplification

An enzyme electrode with NAD amplification for ammonia analysis was constructed by the combination of an oxygen electrode with an immobilized multi-enzyme (NADH oxidase, alcohol dehydrogenase, and NAD synthetase) membrane. The response time of the sensor was measured as one min. and the detection li...

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Bibliographic Details
Published in:Journal of Advanced Science 1998/09/30, Vol.10(2-3), pp.122-123
Main Authors: FUJIMOTO, Yukinobu, HIROTA, Etsuko, KUZUOKA, Youichi, MATSUO, Shinobu, SAWAMOTO, Yuko, YOSHIDA, Ayako, YODA, Kentaro, MISAKI, Hideo
Format: Article
Language:English
Subjects:
Ammonia
Enzyme electrode
NAD amplification
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Description
Summary:An enzyme electrode with NAD amplification for ammonia analysis was constructed by the combination of an oxygen electrode with an immobilized multi-enzyme (NADH oxidase, alcohol dehydrogenase, and NAD synthetase) membrane. The response time of the sensor was measured as one min. and the detection limit of ammonia was as low as 0.2μM.
ISSN:0915-5651
1881-3917
DOI:10.2978/jsas.10.2-3_122