Assay of caffeine and its metabolites in the rat blood plasma using HPLC as a method for determination of metabolic ratios

A technique has been developed for the assay of caffeine and its metabolites in biological liquids (the rat blood plasma). The analysis was carried out using high-performance liquid chromatography with an ultraviolet detector. The limits of quantification (LOQ) for caffeine, paraxanthine, theobromin...

Full description

Saved in:
Bibliographic Details
Published in:Moscow University chemistry bulletin 2013, Vol.68 (1), p.47-50
Main Authors: Novitskaya, Ya. G., Litvin, A. A., Zherdev, V. P., Blynskaya, E. V., Kondakov, S. E.
Format: Article
Language:English
Subjects:
Chemistry
Chemistry and Materials Science
Chemistry/Food Science
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:A technique has been developed for the assay of caffeine and its metabolites in biological liquids (the rat blood plasma). The analysis was carried out using high-performance liquid chromatography with an ultraviolet detector. The limits of quantification (LOQ) for caffeine, paraxanthine, theobromine, and theophylline were calculated to be 10 ng/mL, and the LOQ of 1,3,7-trimethyluric acid was 25 ng/mL.
ISSN:0027-1314
1935-0260
DOI:10.3103/S0027131413010082