Hydrogel nanoparticle encapsulated plasmid as a suitable gene delivery system

To facilitate the delivery of genetic material, the use of appropriate carriers such as polymers is necessary. Nanoparticles comprising of chitosan-alginate polymers were formed through pregel preparation method. Chi/Alg nanoparticles had a mean Z-Average diameter of 161.8 nm and mean zeta 29.3 mV,...

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Bibliographic Details
Published in:Cytology and genetics 2015-03, Vol.49 (2), p.90-94
Main Authors: Rafiee, A., Riazi-Rad, F., Alimohammadian, M. H., Gazori, Taraneh, Fatemi, S. M. R., Havaskary, M.
Format: Article
Language:English
Subjects:
Biomedical and Life Sciences
Biomedicine
Human Genetics
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Summary:To facilitate the delivery of genetic material, the use of appropriate carriers such as polymers is necessary. Nanoparticles comprising of chitosan-alginate polymers were formed through pregel preparation method. Chi/Alg nanoparticles had a mean Z-Average diameter of 161.8 nm and mean zeta 29.3 mV, respectively. The ability of plasmid-complex in preventing DNA migration showed Chi/Alg nanoparticles have great capacity to maintain plasmid. The efficiency of nanoparticles for transfection of pEGFP-N1 plasmid in the cultured HEK 293 cells was measured by flow cytometry. Cell viability assays indicated that nanoparticles had no toxic effect on HEK 293 cells after 4 or 24 h. Our suitable candidate for gene delivery would be alg/chi nanoparticles.
ISSN:0095-4527
1934-9440
DOI:10.3103/S0095452715020097