The homologous identification of the stem rust resistance genes Rpg5, Adf3 and RGA1 in the relatives of barley

• Published in: Cytology and genetics 2016-03, Vol.50 (2), p.96-105
• Main Authors: Ivaschuk, B. V., Samofalova, D. O., Pirko, Ya. V., Fedak, G., Blume, Ya. B.
• Format: Article
• Language: English
• Subjects: Biomedical and Life Sciences; Biomedicine; Human Genetics
• ISSN: 0095-4527; 1934-9440
• DOI: 10.3103/S0095452716020055

The barley genes Rpg5 , RGA1 and Adf3 , which provide a strong resistance to many pathotypes of stem rust, were cloned a few years ago, but it was still unclear whether their homologues were represented in wheat and in related species. The paper describes the results of a bioinformatic research to determine the homologues of Rpg5 , RGA1 and Adf3 in the genomes of Triticum aestivum and several wild grasses, which breeders usually use as sources of stem rust resistance, and which are available in the genome databases. It was found that the Th. elongatum sequence Q9FEC6 and T. aestivum sequence Q43655 were the highly identical homologues of the Adf3 sequence. T. urartu M8A999 sequence and T. aestivum W5FCU1 sequence were found to be the closest homologues of Rpg5 complete protein sequence, but the identity of their kinase domains was not as clear as that of the other domains. The separate Rpg5 kinase part analysis did not provide the strong evidences that its orthologs were present in our corn species. T. urartu M7ZZX9 sequence and T. aestivum W5FFP0 and W5FI33 sequences were shown to be the homologues of RGA1 . The analysis of the predicted active sites allowed finding out the difference between sequences of Rpg5 , RGA1 , Adf3 protein and their homologues.