New phytoviral vector for superexpression of target proteins in plants

Previously, we obtained a new viral vector based on the Alternanthera mosaic virus strain MU (AltMV-MU). The gene of interest was placed under control of two subgenomic promoters (sgp). Viral vector provided the superexpression of target proteins in Nicotiana benthamiana. In the present work, to inc...

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Bibliographic Details
Published in:Moscow University biological sciences bulletin 2013-10, Vol.68 (4), p.169-173
Main Authors: Putlyaev, E. V, Smirnov, A. A, Lazareva, E. A, Klink, G. V, Karpova, O. V, Atabekov, J. G
Format: Article
Language:English
Subjects:
Alternanthera mosaic virus
Biochemistry
Biomedical and Life Sciences
Cell Biology
coat proteins
genes
Life Sciences
Nicotiana benthamiana
plant proteins
Plant Sciences
protein synthesis
Zoology
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Summary:Previously, we obtained a new viral vector based on the Alternanthera mosaic virus strain MU (AltMV-MU). The gene of interest was placed under control of two subgenomic promoters (sgp). Viral vector provided the superexpression of target proteins in Nicotiana benthamiana. In the present work, to increase the level of protein expression, this viral vector was modified and the coat protein gene was placed under control of three sgp. The new viral vector provided superexpression of the protein of interest in plants, and its amount was more than 50% of the total soluble protein in cells. The efficiency of the target protein expression in the plants transformed with viral vectors containing two and three sgp was compared.
ISSN:0096-3925
1934-791X
DOI:10.3103/S0096392513040068