Study of RNA polymerase transcription through nucleosome using the cryo-electron microscopy approach

A promising and reliable method for the analysis of macromolecule structures is capturing images using cryo-electron transmission microscopy and consequently performing three-dimensional reconstructions. In this study, using cryo-electron microscopy, we analyzed the structure of the complex, formed...

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Bibliographic Details
Published in:Moscow University biological sciences bulletin 2016, Vol.71 (1), p.34-38
Main Authors: Volokh, O. I, F. K. Hsieh, M. G. Karlova, E. S. Trifonova, V. M. Studitsky, O. S. Sokolova
Format: Article
Language:English
Subjects:
Biochemistry
Biomedical and Life Sciences
Cell Biology
cryo-electron microscopy
DNA-directed RNA polymerase
Life Sciences
Molecular Biology
nucleosomes
Plant Sciences
Zoology
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Summary:A promising and reliable method for the analysis of macromolecule structures is capturing images using cryo-electron transmission microscopy and consequently performing three-dimensional reconstructions. In this study, using cryo-electron microscopy, we analyzed the structure of the complex, formed by RNA polymerase stalled at position +42 during its transcription through the nucleosome. We obtained both projection images and a three-dimensional structure of the EC-42 complex at 2.5 nm resolution. This allowed us to confirm the conformational integrity of the nucleosome during the passage of the RNA polymerase.
ISSN:0096-3925
1934-791X
DOI:10.3103/S0096392516010120