Screening of the culture media with different concentrations of nutrients for cultivation of the microalgae associated with the invertebrates of the White Sea

• Published in: Moscow University biological sciences bulletin 2016-04, Vol.71 (2), p.102-107
• Main Authors: Lobakova, E. S., Selyakh, I. O., Semenova, L. R., Chivkunova, O. B., Shcherbakov, P. N., Solovchenko, A. E.
• Format: Article
• Language: English
• Subjects: Biochemistry; Biomedical and Life Sciences; Cell Biology; Life Sciences; Methods; Plant Sciences; Zoology
• ISSN: 0096-3925; 1934-791X
• DOI: 10.3103/S009639251602005X

The growth and biomass accumulation of three microalgal strains of Desmodesmus (Scenedesmaceae, Chlorophyceae), 1Рm66В, 2Cl66E, and 3Dp86Е-1, isolated from the White Sea benthic invertebrates were studied under conditions of batch culture in different standard media (BG-11, Prat, Goldberg, Gromov, Tamiya, artificial seawater) and modified media. The culture condition, biomass accumulation, and uptake of nitrate and phosphate were recorded. A significant alkalization of the culture medium up to pH 10 has been observed during a vigorous growth of the microalgae. The most significant biomass accumulation has been recorded in BG-11 (in complete or modified medium with addition of artificial seawater), Tamiya, and Prat media. Addition of seawater did not affect the growth of Desmodesmus sp. in the nitrate-containing media, although that maintained growth of the microalgae in the nitrogen-lacking media without cell aggregation. The BG-11 medium appears suitable for isolation and cultivation of both symbiotic and free-living microalgae by all the tested features. The Prat medium is the best for maintaining the microalgal strains in living collection.