Antioxidant compounds in the seaweed Gelidiella acerosa protects human Peripheral Blood Mononuclear Cells against TCDD induced toxicity

Abstract 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is a persistent environmental toxin formed as an unintentional by-product of incomplete combustion. Several therapeutic approaches have evolved to combat its toxicity since it elicits immunotoxicity, neurotoxicity, hepatotoxicity, carcinogenicity a...

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Published in:Drug and chemical toxicology (New York, N.Y. 1978) N.Y. 1978), 2015-04, Vol.38 (2), p.133-144
Main Authors: Ilavarasi, K., Chermakani, P., Arif Nisha, S., Sheeja Malar, D., Pandima Devi, K.
Format: Article
Language:English
Subjects:
anti-apoptotic
Antioxidant
Antioxidants - isolation & purification
Antioxidants - pharmacology
Apoptosis - drug effects
Chromatography, Liquid - methods
CYP1A1
CYP1B1
Cytochrome P-450 CYP1A1 - genetics
cytoprotection
DNA Damage - drug effects
Environmental Pollutants - toxicity
G. acerosa
Humans
Leukocytes, Mononuclear - drug effects
Leukocytes, Mononuclear - metabolism
Lipid Peroxidation - drug effects
Mass Spectrometry
Membrane Potential, Mitochondrial - drug effects
Polychlorinated Dibenzodioxins - toxicity
Reverse Transcriptase Polymerase Chain Reaction - methods
Rhodophyta - chemistry
TCDD
Up-Regulation - drug effects
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Summary:Abstract 2,3,7,8-Tetrachlorodibenzo-p-dioxin (TCDD) is a persistent environmental toxin formed as an unintentional by-product of incomplete combustion. Several therapeutic approaches have evolved to combat its toxicity since it elicits immunotoxicity, neurotoxicity, hepatotoxicity, carcinogenicity and lethality. Search for drugs from natural resources especially from seaweeds has become intense due to their enormous pharmacological potential. Hence, the present study aims at revealing the protective effect of methanolic extract of G. acerosa (MEGA) in Peripheral Blood Mononuclear Cells (PBMC) against TCDD induced toxicity, by assessing the antioxidant, anti-apoptotic and cytoprotective activities. The results of antioxidant assays suggests that MEGA reverted TCDD induced toxicity by causing an alteration in the levels of antioxidant enzymes (Catalase [CAT], Superoxide dismutase [SOD], Glutathione peroxidase [GPx], Glutathione-S-transferase [GST]) and Glutathione [GSH]. The results of lipid peroxidation assay and protein carbonyl content reveal that MEGA protects PBMC from TCDD induced macromolecular damage. MEGA was found to exhibit significant (p 
ISSN:0148-0545
1525-6014
DOI:10.3109/01480545.2014.919582