The in vitro genotoxic and cytotoxic effects of remeron on human peripheral blood lymphocytes

• Published in: Drug and chemical toxicology (New York, N.Y. 1978) N.Y. 1978), 2015-07, Vol.38 (3), p.266-271
• Main Authors: Norizadeh Tazehkand, Mostafa, Topaktas, Mehmet
• Format: Article
• Language: English
• Subjects: Adrenergic alpha-Antagonists - toxicity; Adult; Cell Proliferation - drug effects; Cells, Cultured; Chromosome aberration; cytotoxicity; Dose-Response Relationship, Drug; Female; genotoxicity; human lymphocytes; Humans; Lymphocytes - drug effects; Lymphocytes - pathology; Male; Mianserin - analogs & derivatives; Mianserin - toxicity; Micronuclei, Chromosome-Defective - chemically induced; micronucleus; Micronucleus Tests; Mirtazapine; Mitosis - drug effects; Mitotic Index; remeron; Risk Assessment; Serotonin Antagonists - toxicity; sister chromatid exchange; Sister Chromatid Exchange - drug effects; Time Factors; Young Adult
• ISSN: 0148-0545; 1525-6014
• DOI: 10.3109/01480545.2014.947425

Remeron (Mirtazapine) is an antidepressant drug which exerts its action by blocking presynaptic α-2-adrenergic receptors and postsynaptic serotonin types 2 and 3 receptors. In this in vitro analysis, human peripheral blood lymphocytes was treated by remeron (10, 25, 40 and 55 μg/mL) for 24 hours and 48 hours periods, then it was attempted to study of genotoxic and cytotoxic effects of the substance on human peripheral blood lymphocytes by some tests such as sister chromatid exchange (SCE), chromosomal abnormalities (CA) and micronucleus (MN) tests. Also proliferating effect of the substance was investigated. Remeron didn't significantly cause chromosomal abnormalities and sister chromatid exchange while caused micronucleus at 40 μg/mL concentration and 24 h periodic time and increased proliferation index of the both 24 and 48 hours treated cells was decreased in a concentration manner. Also, exposing to the remeron for 24 and 48 hours leaded to a decrease in mitotic index and nucleus division index in the cells by concentration dependent manner. These findings showed that remeron did not have significantly genotoxic effects on human peripheral blood lymphocytes while it showed cytotoxic effects on the cells, which is the first report on genotoxic and cytotoxic properties of remeron.