Infliximab attenuates activated charcoal and polyethylene glycol aspiration-induced lung injury in rats

• Published in: Experimental lung research 2012-04, Vol.38 (3), p.147-156
• Main Authors: Güzel, Aygül, Günaydin, Mithat, Güzel, Ahmet, Alaçam, Hasan, Murat, Naci, Gacar, Ayhan, Güvenç, Tolga
• Format: Article
• Language: English
• Subjects: activated charcoal; Animals; Antibodies, Monoclonal - pharmacology; aspiration; Charcoal - toxicity; Gastrointestinal Agents - adverse effects; Glutathione Peroxidase - metabolism; Infliximab; Lung - drug effects; Lung - metabolism; lung injury; Lung Injury - chemically induced; Lung Injury - drug therapy; Lung Injury - metabolism; Male; Malondialdehyde - metabolism; Nitric Oxide Synthase Type II - metabolism; Oxidative Stress - drug effects; Pneumonia - drug therapy; Pneumonia - metabolism; polyethylene glycol; Polyethylene Glycols - toxicity; Pulmonary Surfactant-Associated Protein D - metabolism; Rats; Rats, Sprague-Dawley; surfactant protein-D; TNF-α; Tumor Necrosis Factor-alpha - antagonists & inhibitors; Tumor Necrosis Factor-alpha - metabolism
• ISSN: 0190-2148; 1521-0499
• DOI: 10.3109/01902148.2012.659836

ABSTRACT Aspiration is a serious complication of gastrointestinal (GI) decontamination procedure. Studies have shown that tumor necrosis factor-α (TNF-α) blockers have beneficial effects on lung injury. Therefore, the authors investigated the attenuation by infliximab (INF) on activated charcoal (AC)- and polyethylene glycol (PEG)-induced lung injury in rat model. Forty-two male Sprague-Dawley rats were allotted into 1 of 6 groups: saline (NS), activated charcoal (AC), polyethylene glycol (PEG), NS+INF treated, AC+INF treated, and PEG+INF treated. All materials were aspirated into the lungs at a volume of 1 mL/kg. Before aspiration, the rats were injected subcutaneously with INF. Seven days later, both lungs and serum specimens in all groups were evaluated histopathologically, immunohistochemically, and biochemically. Following aspiration of AC and PEG, evident histopathological changes were assigned in the lung tissue that were associated with increased expression of inducible nitric oxide synthase (iNOS), increased serum levels of oxidative stress markers (malondialdehyde [MDA], surfactant protein-D [SP-D], TNF-α), and decreased antioxidant enzyme (glutathione peroxidase [GSH-Px]) activities. INF treatment significantly decreased the elevated serum MDA and TNF-α levels and increased serum GSH-Px levels. Furthermore, the current results show that there is a significant reduction in the activity of iNOS in lung tissue and increased serum SP-D levels of AC and PEG aspiration-induced lung injury with INF treatment. These findings suggest that INF attenuates lung inflammation and prevents GI decontamination agent-induced lung injury in rats.