Development of chitosan-pullulan composite nanoparticles for nasal delivery of vaccines: optimisation and cellular studies

Nasal immunisation with nanoparticles has already shown promising results. In this study, nanoparticle composites carrying BSA for nasal vaccination prepared using electrostatic interaction process between polycation N-trimethyl chitosan chloride (TMC), chitosan glutamate (CG), chitosan chloride (CC...

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Bibliographic Details
Published in:Journal of microencapsulation 2015-11, Vol.32 (8), p.755-768
Main Authors: Cevher, Erdal, Salomon, Stefan K., Makrakis, Apostolos, Li, Xiong Wei, Brocchini, Steve, Alpar, H. Oya
Format: Article
Language:English
Subjects:
Administration, Intranasal
Animals
Carboxymethyl pullulan
Cell Line
cellular uptake
Chitosan - chemistry
Chitosan - pharmacokinetics
Chitosan - pharmacology
chitosan derivatives
Glucans - chemistry
Glucans - pharmacokinetics
Glucans - pharmacology
Mice
N-trimethyl chitosan chloride
Nanocomposites - chemistry
Nanocomposites - ultrastructure
nanoparticles
nasal vaccination
Particle Size
polyion complexation
Vaccines - chemistry
Vaccines - pharmacokinetics
Vaccines - pharmacology
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Summary:Nasal immunisation with nanoparticles has already shown promising results. In this study, nanoparticle composites carrying BSA for nasal vaccination prepared using electrostatic interaction process between polycation N-trimethyl chitosan chloride (TMC), chitosan glutamate (CG), chitosan chloride (CCl) and polyanion carboxymethyl pullulan (CMP). A mass ratio of 2:1 for TMC-CMP combination produced stable nanocarriers. For CCl-CMP and CG-CMP formulations needed a mass ratio of 3:1. Loading efficiency was >90% for all formulations. Nanoparticles' size ranged from 207 to 603 nm. The surface charge of the complexes varied between +14 and +33 mV. SDS-PAGE integrity of the model antigen was also demonstrated. MTT studies showed that nanoparticle composites were less toxic to Calu-3 cells than the particles of cationic polymers alone. FITC-BSA loaded nanoparticles efficiently taken up by J774A.1 macrophages as confirmed by confocal microscopy highlighting the potential of these novel nanoparticulate carriers' use for nasal vaccination.
ISSN:0265-2048
1464-5246
DOI:10.3109/02652048.2015.1073392