Biotransformation of 3β-hydroxy-5-en-steroids by Mucor silvaticus

Abstract The biotransformation of four 3β-hydroxy-5-en-steroids with varying substituents at C-16 or/and C-17 by Mucor silvaticus was investigated. The characterization of the metabolites was performed by IR, MS, 1H NMR, 13C NMR, and 2-D NMR. All the examined substrates were transformed, mainly by 7...

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Bibliographic Details
Published in:Biocatalysis and biotransformation 2013-08, Vol.31 (4), p.168-174
Main Authors: Wang, Yanjie, Sun, Dongmei, Chen, Zhibao, Ruan, Hongsheng, Ge, Wenzhong
Format: Article
Language:English
Subjects:
biotransformation
hydroxylation
Mucor silvaticus
steroids
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Summary:Abstract The biotransformation of four 3β-hydroxy-5-en-steroids with varying substituents at C-16 or/and C-17 by Mucor silvaticus was investigated. The characterization of the metabolites was performed by IR, MS, 1H NMR, 13C NMR, and 2-D NMR. All the examined substrates were transformed, mainly by 7α-hydroxylation. Studies carried out with M. silvaticus demonstrated the versatility of this organism in introducing hydroxyl groups at the 7α-, 9α-, 11α-, and 14α-positions in 3-ol-5-ene steroids. The relationships between the substrate structures and hydroxylated positions are also discussed.
ISSN:1024-2422
1029-2446
DOI:10.3109/10242422.2013.813490