Molecular Cloning and Sequence Analysis of Bubaline Lactoferrin Promoter

The proximal promoter for bubaline lactoferrin-encoding gene has been isolated, cloned and sequenced. A 468 b fragment of the 5′ flanking region of the Pactoferrin gene was PCR amplified and cloned into pUC18 vector. Sequence analysis of the amplified fragment revealed the presence of one TATA box,...

Full description

Saved in:
Bibliographic Details
Published in:DNA sequence 1999, Vol.10 (2), p.97-99
Main Authors: Das, Paramananda, Tiwari, Gunjan, Rupa, Prithy, Garg, Lalit C.
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Buffaloes - genetics
Cattle
Cloning, Molecular
Humans
Lactoferrin
Lactoferrin - genetics
Mice
Molecular Sequence Data
PCR
promoter
Promoter Regions, Genetic
Swine
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The proximal promoter for bubaline lactoferrin-encoding gene has been isolated, cloned and sequenced. A 468 b fragment of the 5′ flanking region of the Pactoferrin gene was PCR amplified and cloned into pUC18 vector. Sequence analysis of the amplified fragment revealed the presence of one TATA box, one TATA like element, two GC boxes and one motif resembling CAMP response element (CRE) in this reion. Bubaline lactoferrin romoter shares 93%, 53%, 52% and 48% homoPogy with cattle, pig, mouse and human lactoferrin 5′ flanking region, respectively.
ISSN:1042-5179
1029-2365
DOI:10.3109/10425179909008424