Intracellular drug delivery in Leishmania-infected macrophages: Evaluation of saponin-loaded PLGA nanoparticles

Drug delivery systems present an opportunity to potentiate the therapeutic effect of antileishmanial drugs. Colloidal carriers are rapidly cleared by the phagocytic cells of the reticuloendothelial system (RES), rendering them ideal vehicles for passive targeting of antileishmanials. This paper desc...

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Bibliographic Details
Published in:Journal of drug targeting 2012-02, Vol.20 (2), p.142-154
Main Authors: Van de Ven, H., Vermeersch, M., Vandenbroucke, R.E., Matheeussen, A., Apers, S., Weyenberg, W., De Smedt, S.C., Cos, P., Maes, L., Ludwig, A.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cell Line
co-localisation
Drug Delivery Systems - methods
Escin - administration & dosage
Escin - pharmacokinetics
Escin - pharmacology
General pharmacology
GFP-transfected L. donovani
J774A.1 macrophages
Lactic Acid - chemistry
Leishmania infantum - drug effects
Leishmaniasis
Macrophages - drug effects
Macrophages - microbiology
Medical sciences
Mice
Microbial Sensitivity Tests - methods
Microbial Sensitivity Tests - statistics & numerical data
Nanoparticles - administration & dosage
Nanoparticles - chemistry
Pharmaceutical technology. Pharmaceutical industry
Pharmacology. Drug treatments
PLGA nanoparticles
Polyglycolic Acid - chemistry
saponins
simplex mixture design
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Summary:Drug delivery systems present an opportunity to potentiate the therapeutic effect of antileishmanial drugs. Colloidal carriers are rapidly cleared by the phagocytic cells of the reticuloendothelial system (RES), rendering them ideal vehicles for passive targeting of antileishmanials. This paper describes the development of poly(D,L-lactide-co-glycolide) (PLGA) nanoparticles (NPs) for the antileishmanial saponin β-aescin. NPs were prepared using the combined emulsification solvent evaporation/salting-out technique. Confocal microscopy was used to visualise the internalisation and intracellular trafficking of fluorescein- and nile red-labelled PLGA NPs in J774A.1 macrophages infected with GFP-transfected Leishmania donovani. The in vitro activity of aescin and aescin-loaded NPs on L. infantum was determined in the axenic model as well as in the ex vivo model. The developed PLGA NPs were monodispersed with Zave
ISSN:1061-186X
1029-2330
DOI:10.3109/1061186X.2011.595491