Recombinant PTD-Cu/Zn SOD attenuates hypoxia-reoxygenation injury in cardiomyocytes

• Published in: Free radical research 2013-05, Vol.47 (5), p.386-393
• Main Authors: Liu, J., Hou, J., Xia, Z. Y., Zeng, W., Wang, X., Li, R., Ke, C., Xu, J., Lei, S., Xia, Z.
• Format: Article
• Language: English
• Subjects: Animals; Antioxidants - metabolism; Apoptosis; cardiomyocyte; Cells, Cultured; Copper/zinc superoxide dismutase; Gene Expression Regulation - drug effects; Humans; Hydrogen Peroxide - metabolism; Hypoxia - metabolism; Hypoxia - pathology; hypoxia-reoxygenation injury; Mitochondria - metabolism; Mitochondria - pathology; Myocardial Reperfusion Injury - metabolism; Myocardial Reperfusion Injury - pathology; Myocytes, Cardiac - cytology; Myocytes, Cardiac - drug effects; Oxidative Stress - drug effects; Protein transduction domain; Rats; Reactive Oxygen Species - metabolism; Recombinant Fusion Proteins - administration & dosage; Recombinant Fusion Proteins - genetics; Superoxide Dismutase - administration & dosage; Superoxide Dismutase - genetics; Superoxides - metabolism
• ISSN: 1071-5762; 1029-2470
• DOI: 10.3109/10715762.2013.780286

Abstract Background. Oxidative stress plays a pivotal role in myocardial ischemia-reperfusion injury. Increasing the protein expression of intracellular Cu/Zn SOD, which is the major endogenous antioxidant enzyme, may attenuate or prevent hypoxia-reoxygenation injury (HRI) in cultured cardiomyocytes. However, ectogenic Cu/Zn-SOD can hardly be transferred into cells to exert biological effects. In this study, we constructed PTD-Cu/Zn SOD plasmid with a kind of translocation structure-Protein transduction domain (PTD) and detected its transmembrane ability and antioxidant effects in H9c2 rat cardiomyocytes subjected to hypoxia/reoxygenation injury (HRI). Methods. We constructed the pET-PTD-Cu/Zn SOD (CDs) prokaryotic expression vectors in plasmid that were inserted into E. coli BL21 to induce the protein expression of PTD-Cu/Zn SOD. H9c2 cardiomyocyte HRI was achieved by exposing cardiomyocytes to 12 h hypoxia followed by 2 h reoxygenation. Protein expression of PTD-Cu/Zn SOD in cardiomyocytes was assayed by Western blot and their enzyme activities were investigated by immunohistochemistry and flow cytometry. Results. In cultured cardiomyocytes hypoxia-reoxygenation injury model, exogenous PTD-Cu/Zn SOD could penetrate cell membrane to clear superoxide anion and decrease hydrogen peroxide level in H9c2 cardiomyocytes subjected to HRI. The level of mitochondrial membrane potential was restored to normal, and the cell apoptosis was reduced in cardiomyocytes with PTD-Cu/Zn SOD treatment during HRI. Conclusion. Recombinant PTD-Cu/Zn SOD could scavenge intracellular-free superoxide anion, protect mitochondria from damages, and attenuate the hypoxia-reoxygenation injury in cultured cardiomyocytes.