Daily or monthly ibandronate prevents or restores deteriorations of bone mass, architecture, biomechanical properties and markers of bone turnover in androgen-deficient aged rats

• Published in: The aging male 2011-12, Vol.14 (4), p.220-230
• Main Authors: De La Piedra, Concepcion, Quiroga, I., Montero, M., Dapia, S., Caeiro, J. R., Rubert, M., Diaz-Curiel, M., Bauss, F.
• Format: Article
• Language: English
• Subjects: Acid Phosphatase - blood; androgen deficiency; Androgens; Androgens - deficiency; Animals; Biomarkers; Biomechanical Phenomena; Biomechanics; Bone and Bones - drug effects; Bone Density - drug effects; Bone Density Conservation Agents - administration & dosage; Bone Resorption - drug therapy; Diphosphonates - administration & dosage; Femur - drug effects; Ibandronate; intermittent treatment; Isoenzymes - blood; Male; male osteoporosis; Males; Orchiectomy; Osteocalcin - blood; Osteoporosis; Peptide Fragments - blood; Procollagen - blood; Rats; Rodents; Tartrate-Resistant Acid Phosphatase
• ISSN: 1368-5538; 1473-0790
• DOI: 10.3109/13685538.2010.518176

Aim. The aim of this study was to investigate the effects of the bisphosphonate ibandronate (IBN) in a male osteoporosis animal model. Methods. Two studies were performed in 9-month-old orchidectomised (ORX) or sham-operated rats. In prevention study, subcutaneous IBN was administered daily (1 μg/kg) or monthly (28 μg/kg every 28 days) starting on day of surgery for 5 months. In treatment study, the same treatment started 6 months after ORX. After sacrifice, bone analyses by dual-energy X-ray absorptiometry, 3-dimensional micro-computed tomography, and 3-point bending were performed in femora or vertebrae. Serum tartrate-resistant acid phosphatase 5b (TRAP-5b) and aminoterminal propeptide of collagen I (PINP) were analysed for resorption and osteocalcin (BGP) for bone formation. Results. In both studies, ORX resulted in significant femoral and vertebral bone loss and microarchitectural deterioration after 5 months of ORX, and became more pronounced after 11 months. Biomechanical strength was also decreased. Serum levels for TRAP-5b and BGP increased while PINP levels were reduced or unchanged. Both daily and monthly IBN prevented or even restored ORX-induced changes in both studies, with the intermittent regimen showing a improvement in efficacy with respect to many of the biomechanical parameters.