Reactivation of immobilized acetylcholinesterasetabun complex by pralidoxime, its isomers, and homologs

Reactivation efficacy of three homologous and three isomeric series of pralidoxime-type reactivators with aldoxime group in position 2, 3 and 4 of the heterocycle was tested in reactivation of tabun-inhibited AChE. The experiments were performed with immobilized and stabilized porcine brain AChE. Th...

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Bibliographic Details
Published in:Toxicology mechanisms and methods 2010-06, Vol.20 (5), p.223-226
Main Authors: Hoskovcová, Monika, Halámek, Emil, Kobliha, Zbyn k, Tušarová, Ivana
Format: Article
Language:English
Subjects:
Acetylcholinesterase
Brain
Ellman reagent
immobilised enzyme
pralidoxime
reactivation
tabun
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Summary:Reactivation efficacy of three homologous and three isomeric series of pralidoxime-type reactivators with aldoxime group in position 2, 3 and 4 of the heterocycle was tested in reactivation of tabun-inhibited AChE. The experiments were performed with immobilized and stabilized porcine brain AChE. The enzyme acticity was measured by Ellman method. Reactivation efficacy was determined by measurement of indicator fabric coloration intensity as a measure of AChE activity. Of the studied group of nine reactivators, isomers with the functional group in position 2 were the most effective. The highest value (30 %) for reactivation of inhibited AChE was found for 2PAE after treatment for 15 min at concentration 0.5 mg/cm3. The efficacy of the isomers decreased in the order ortho > para > meta. No marked effect on the efficacy of the reactivators was observed on prolongation of the reactivation time. The reactivators efficacy decreased with decreasing concentration of their solutions.
ISSN:1537-6516
1537-6524
DOI:10.3109/15376511003746026