The effect of methimazole-induced postnatal hypothyroidism on the retinal maturation and on the Sirtuin 2 level

• Published in: Cutaneous and ocular toxicology 2016-01, Vol.35 (1), p.36-40
• Main Authors: Kocaturk, Tolga, Ergin, Kemal, Cesur, Gokhan, Evlicoglu, Gokhan Evren, Cakmak, Harun
• Format: Article
• Language: English
• Subjects: Animals; Animals, Newborn; Antithyroid Agents; Female; Hypothyroidism - chemically induced; Hypothyroidism - metabolism; Methimazole; postnatal hypothyroidism; Rats, Wistar; Retina - anatomy & histology; Retina - drug effects; Retina - metabolism; retinal maturation; Sirtuin 2; Sirtuin 2 - metabolism
• ISSN: 1556-9527; 1556-9535
• DOI: 10.3109/15569527.2015.1007509

Purpose: To investigate the effect of methimazole-induced postnatal hypothyroidism on the retinal maturation and to study Sirtuin 2 (SIRT2) level in the hypothyroidic rat retina. Methods: Twenty newborn Wistar albino rat pups were used in this prospective, randomized study. Wistar albino rats, weight 250-300 g, were impregnated (without addition of any drug) and were fed normally. Rat pups were randomly divided into two groups and were fed with breast milk. After weaning till they were 90 days of age, rat pups received the same water as their lactating mothers drank. Group 1 (methimazole (MMI)-induced hypothyroidy group), rats were given MMI-water, whereas, in Group 2, normal tap water. When the pups were 90 days of age, 20 rat pups were decapitated and the eyes were isolated. Eyes were investigated using histological, histomorphometric and immunohistochemistrical techniques. Results: No histological difference was seen between the groups stained with hematoxylin and eosin. In both groups the retinal layer structures and cells were observed as normal. The examples in the groups had a normal distribution for retinal thickness (pixel) measure. The mean value (mean ± std. deviation) was 554.7 ± 228.4 in the control group and 494.7 ± 129.4 in the hypothyroidy group. There was no significance between the groups in terms of retinal thickness (p = 0.231). However, immunohistochemistry revealed that SIRT2 was weaker stained in the ganglion cell layer and visual cell layer in the hypothyroidy group compared to the control group. Conclusion: Postnatal hypothyroidism altered the retinal cytoarchitecture and layering which are regulated by thyroid hormones (THs) during retinal maturation in the postnatal period. THs may act by the induction of the SIRT family proteins or through their receptors. Postnatal screenings for THs levels are very important to provide normal retinal development.