Evaluation of yogurt and various beverages as carriers of lactic acid bacteria producing 2-branched (1,3)-β-d-glucan

• Published in: Journal of dairy science 2011-07, Vol.94 (7), p.3271-3278
• Main Authors: Elizaquível, P., Sánchez, G., Salvador, A., Fiszman, S., Dueñas, M.T., López, P., Fernández de Palencia, P., Aznar, R.
• Format: Article
• Language: English
• Subjects: Animal productions; Animals; beta-Glucans - metabolism; Beverages - microbiology; Biological and medical sciences; Citrus sinensis; Food industries; Food Microbiology; Fundamental and applied biological sciences. Psychology; Hydrogen-Ion Concentration; juice; lactic acid bacteria; Lactobacillus - physiology; Microbial Viability; Milk - microbiology; Pediococcus - classification; Pediococcus - physiology; probiotic; Probiotics; Terrestrial animal productions; Vertebrates; yogurt; Yogurt - microbiology
• ISSN: 0022-0302; 1525-3198
• DOI: 10.3168/jds.2010-4026

Probiotic cultures are increasingly being incorporated into a wide variety of food products. Although lactobacilli and bifidobacteria are the most frequently used, other lactic acid bacteria (LAB) have been reported to be potential probiotics. Of these, the cider isolates Pediococccus parvulus (strains 2.6 and CUPV22) and Lactobacillus suebicus CUPV221 produce a 2-branched (1,3)-β-d-glucan exopolysaccharide that decreases serum cholesterol levels and affects the activation of human macrophages. For this reason, these 3 strains were incorporated into yogurt, orange juice, and 2 juice-milk beverages to evaluate the effect of the food matrix on the resistance of these strains to simulated gastrointestinal tract conditions. Our results showed that incorporation of the LAB did not significantly affect the physical and rheological properties of the food matrices tested. When incorporated in yogurt, LAB strains population decreased by 2 to 3 log orders of magnitude during the shelf life of the product (28 d). However, no significant decrease was observed in the juice and juice-milk beverages during the same storage period, except for Lb. suebicus, whose viability decreased by 3 log orders of magnitude. When strains were subjected to gastrointestinal tract conditions, a decrease in the survival was observed at the lower pH (1.8). However, incorporation of these LAB strains into orange juice increases their resistance to lower pH conditions, thus improving survival to gastrointestinal stress. Moreover, a protective effect was observed for P. parvulus CUPV22 and 2.6 to gastric stress in juice-milk beverages and to gastrointestinal stress in yogurt. Lactobacillus suebicus CUPV221 did not survive when incorporated into yogurt and juice-milk beverage.