Short communication: Diversity of staphylococci isolated from sheep mastitis in northern Algeria

• Published in: Journal of dairy science 2020-01, Vol.103 (1), p.890-897
• Main Authors: Achek, Rachid, El-Adawy, Hosny, Hotzel, Helmut, Tomaso, Herbert, Ehricht, Ralf, Hamdi, Taha Mossadak, Azzi, Omar, Monecke, Stefan
• Format: Article
• Language: English
• Subjects: Algeria; Animals; Bacterial Toxins - genetics; Enterotoxins - genetics; Female; Leukocidins - genetics; Mastitis - microbiology; Mastitis - veterinary; Methicillin - pharmacology; microarray; Milk - microbiology; Sheep; Sheep Diseases - microbiology; sheep mastitis; Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization - veterinary; Staphylococcal Infections - microbiology; Staphylococcal Infections - veterinary; Staphylococcus; Staphylococcus - genetics; Staphylococcus - isolation & purification; Staphylococcus - pathogenicity; Superantigens - genetics; Virulence - genetics
• ISSN: 0022-0302; 1525-3198
• DOI: 10.3168/jds.2019-16583

Mastitis in ruminants is an important disease with major effects on both the economy and animal welfare. It is caused by major pathogens such as Staphylococcus aureus and minor pathogens such as coagulase-negative staphylococci. The objective of this study was to identify and characterize staphylococci as a cause of sheep mastitis in Algeria. In this study, 123 milk samples were collected directly from the udder of sheep suffering from clinical mastitis in 2 provinces in Algeria. Recovered isolates were identified using MALDI-TOF mass spectrometry. Virulence-associated and antimicrobial resistance genes as well as clonal complexes (CC) of S. aureus were determined using microarray-based analysis. A total of 45 staphylococci isolates were cultivated from sheep milk samples, and 28 S. aureus were identified as methicillin susceptible (62.2%). Seventeen other Staphylococcus isolates of different species were identified using MALDI-TOF mass spectrometry. Subsequent microarray analysis typed the methicillin-susceptible S. aureus to 6 CC: CC8-MSSA, CC97-MSSA, CC130/521-MSSA, CC479-MSSA, CC522-MSSA, and CC705-MSSA. The accessory gene regulator agrIII and the ruminant leukocidin genes lukF-P83 and lukM were found in all isolates of CC130/521, CC479, CC522, and CC705. The toxic shock syndrome toxin gene tst1 was detected exclusively in CC130/521. Additionally, virulence-associated genes (sea, sed, sak, hld, hlgA, edinB, and others) were detected. The presence of antibiotic resistance genes [blaZ, erm(B), and tet(K)] was detected in small numbers of staphylococci. Staphylococci possessing these genes are considered potential hazards for farm animals, farmers, and consumers. Data concerning the prevalence and diversity of staphylococci causing mastitis in sheep from Algeria are lacking. Presented results on different aspects about staphylococci in Algerian sheep populations should at least partially close that gap. However, further extensive studies covering more geographical regions are needed to assess the epidemiological risk.