Short communication: A pilot study to describe duodenal and ileal flows of nutrients and to estimate small intestine endogenous protein losses in weaned calves

• Published in: Journal of dairy science 2020-10, Vol.103 (10), p.9102-9109
• Main Authors: Ansia, I., Stein, H.H., Brøkner, C., Drackley, J.K.
• Format: Article
• Language: English
• Subjects: Amino Acids - metabolism; Animal Feed; Animals; Catheterization - veterinary; Cattle - metabolism; Diet - veterinary; Dietary Carbohydrates - metabolism; Dietary Proteins - metabolism; digesta flows; digestibility; Digestion; Duodenum - metabolism; endogenous protein; Gastrointestinal Motility; Glycine max - metabolism; Ileum - metabolism; Ileum - surgery; Intestinal Absorption; Intestine, Small - metabolism; Male; Nitrogen - metabolism; Nutrients - metabolism; Pilot Projects; Rumen - metabolism; Soybean Proteins - metabolism; weaned calves; Weaning
• ISSN: 0022-0302; 1525-3198
• DOI: 10.3168/jds.2020-18281

The aims of this pilot study were (1) to evaluate the effect of an ileal and duodenal cannulation surgery on body weight and dry matter intake, (2) to estimate endogenous losses of crude protein (CP) and AA in the small intestine, and (3) to describe duodenal and ileal flows of nutrients in weaned dairy calves. Three Holstein male calves were fitted at 7 wk of life with a T-cannula at the terminal ileum and another cannula at the proximal duodenum. On wk 14 of life, calves were randomly assigned to a single 3 × 3 Latin square with 10-d periods. The 3 diets were fed ad libitum and consisted of a control calf starter (CS) with conventional soybean meal (SBM) as the main source of protein (CTRL), an isonitrogenous (20% CP) CS with an enzyme-treated SBM as the main source of protein (ENZT), and a CS with low content of CP (10%) and no soy protein (LOCP). Flows and digestibilities of nutrients were compared between the soy-based high-protein diets (HICP) and LOCP, and between CTRL and ENZT. Final data were only available from 2 calves per diet (n = 2) because cannulas from 1 calf became inoperative after the first collection period. Duodenal flows of CP, total AA, nonprotein nitrogen, microbial N, and fatty acids, as well as apparent duodenal digestibility of starch, were greater for HICP than for LOCP, indicating a greater foregut microbial activity and digestion. The apparent ileal digestibility (AID) of organic matter, CP, and total AA were greater for HICP than for LOCP. Duodenal net flow of CP was greater for ENZT than for CTRL, but flow of AA was not different. On the other hand, duodenal flow of microbial N was greater, and flows of nonprotein N and starch were lower for the ENZT diet, suggesting a more efficient microbial activity in the rumen. Even though CTRL had a greater AID when compared with ENZT, the AID of CP and AA were greater for enzyme-treated SBM than for SBM. Endogenous losses in the small intestine per kilogram of duodenal dry matter flow were 47 ± 15 and 37 ± 12 g/d, and the true ileal digestibilities for the HICP diets were 86 ± 0.1 and 87 ± 0.1% for CP and AA, respectively. An optimal supply of CP and the inclusion of an enzyme-treated SBM improved the efficiency of microbial digestion and increased AA absorption. Although further research with greater biological replication is needed, our results indicate that there is potential to improve digestion and absorption of proteins through dietary strategies in young weaned calves.