Effects of Vitamin E Supplementation on Antioxidant, Inflammatory Biomarker, and Cell Viability of Peripheral Blood Mononuclear Cells from Japanese Black Calves with or without Lipopolysaccharide Stimulation

The purpose of this study was to determine the effects of vitamin E supplementation on antioxidant, inflammatory status, and cell viability of peripheral blood mononuclear cells. Eighteen clinically healthy Japanese Black calves were used in this study. The peripheral blood mononuclear cells (PBMCs)...

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Bibliographic Details
Published in:Journal of Nutritional Science and Vitaminology 2022/10/31, Vol.68(5), pp.470-474
Main Authors: OTOMARU, Konosuke, MIYAHARA, Takuro, SAITA, Hiroto, MAEDA, Yosuke
Format: Article
Language:English
Subjects:
cell viability
inflammation
Japanese Black calf
peripheral blood mononuclear cells
vitamin E
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Summary:The purpose of this study was to determine the effects of vitamin E supplementation on antioxidant, inflammatory status, and cell viability of peripheral blood mononuclear cells. Eighteen clinically healthy Japanese Black calves were used in this study. The peripheral blood mononuclear cells (PBMCs) were isolated from their venous blood. PBMCs were cultured with vitamin E (vitamin E group) or without vitamin E (control group), and stimulated with or without lipopolysaccharide (LPS). As a result, the total antioxidant capacities, which are the reducing power of components from Fe3+ to Fe 2+, in the cell culture supernatant with or without LPS stimulation were significantly higher in the vitamin E group than that in the control group (p
ISSN:0301-4800
1881-7742
DOI:10.3177/jnsv.68.470