LIPASE PRODUCTION FROM WASTE FRUIT PEELS FOR BIODIESEL PRODUCTION

Waste pineapple and passion fruit peels have potency as lipase enzyme sources. The combination of both peels with a ratio of 6:4 has the highest enzyme lipase activity after 3 days of fermentation and it has increased to 28.33 ± 0.0 U/ml after purification using the ammonium sulfate precipitation me...

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Bibliographic Details
Published in:Rasāyan journal of chemistry 2023-04, Vol.16 (2), p.636-641
Main Authors: Sitepu, E. K., F. Sebayang, Zaidar, E.F., W. Ardilla, M. Antika, Sari, R.N., S. Perangin-angin, Tarigan, J.B.
Format: Article
Language:English
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Summary:Waste pineapple and passion fruit peels have potency as lipase enzyme sources. The combination of both peels with a ratio of 6:4 has the highest enzyme lipase activity after 3 days of fermentation and it has increased to 28.33 ± 0.0 U/ml after purification using the ammonium sulfate precipitation method. The response surface method design experiment was applied to determine the effect of the ratio of molar oil to methanol (1:3 – 1:9), reaction time (1 – 24 hours), and temperature (30 - 60C). The maximum biodiesel conversion of 82.7% could be achieved using the reaction condition of a ratio molar of 1:9, 13.65 hours reaction time, and a temperature of 46.5C for enzymatic transesterification of PO. Further, for bio-catalysis biodiesel production from WCO, the maximum conversion of 88% was achieved under reaction conditions of a ratio molar of 1:9, 14 hours reaction time, and a temperature of 48C.
ISSN:0974-1496
0974-1496
DOI:10.31788/RJC.2023.1628216