Evaluation of a range of doses of ultraviolet irradiation to inactivate waterborne actinospore stages of Myxobolus cerebralis

The ability of a range of doses of ultraviolet irradiation (UV) to inactivate the waterborne actinospore or triactinomyxon stages (TAMs) of Myxobolus cerebralis was evaluated by infectivity for juvenile rainbow trout Oncorhynchus mykiss. TAMs were UV-irradiated using a low pressure mercury vapour la...

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Bibliographic Details
Published in:Diseases of aquatic organisms 2007-02, Vol.74 (2), p.113-118
Main Authors: HEDRICK, R. P, PETRI, B, MCDOWELL, T. S, MUKKATIRA, K, SEALEY, L. J
Format: Article
Language:English
Subjects:
Animal aquaculture
Animal productions
Animals
Biological and medical sciences
Dose-Response Relationship, Radiation
Eukaryota - pathogenicity
Eukaryota - radiation effects
Fish Diseases - parasitology
Fundamental and applied biological sciences. Psychology
Oncorhynchus mykiss
Pisciculture
Protozoan Infections, Animal - parasitology
Spores, Protozoan - pathogenicity
Spores, Protozoan - radiation effects
Time Factors
Ultraviolet Rays
Vertebrate aquaculture
Water - parasitology
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Summary:The ability of a range of doses of ultraviolet irradiation (UV) to inactivate the waterborne actinospore or triactinomyxon stages (TAMs) of Myxobolus cerebralis was evaluated by infectivity for juvenile rainbow trout Oncorhynchus mykiss. TAMs were UV-irradiated using a low pressure mercury vapour lamp collimated beam apparatus. All doses 40, 80, 120 and 160 mJ cm(-2) were found to completely inactivate the TAMs as demonstrated by the absence of microscopic lesions, myxospores and parasite DNA detected by quantitative PCR (qPCR) among rainbow trout 5 mo post-exposure. In contrast, rainbow trout receiving the same concentrations of untreated TAMs (1000 fish(-1)) developed clinical signs of whirling disease at 2 mo post-exposure and had severe microscopic lesions, high myxospore counts and high qPCR values when examined at 5 mo following exposure to the parasite.
ISSN:0177-5103
1616-1580
DOI:10.3354/dao074113