Antioxidant and Metal-Chelating Activities of Bioactive Peptides from Ovotransferrin Produced by Enzyme Combinations

• Published in: Poultry 2022-12, Vol.1 (4), p.220-228
• Main Authors: Wickramasinghe, Hiruni, Abeyrathne, Edirisinghe, Nam, Ki-Chang, Ahn, Dong
• Format: Article
• Language: English
• ISSN: 2674-1164; 2674-1164
• DOI: 10.3390/poultry1040019

Peptides produced from food sources possess numerous bioactivities that make them useful in improving human health and preventing diseases. Although many studies related to egg protein hydrolysis are available, little work has been conducted on the production of bioactive peptides from apo-ovotransferrin (OTF) using two-step enzyme hydrolysis. The objectives of this study were to produce bioactive peptides from OTF using two enzymes and to determine their functional properties. Lipolyzed OTF was prepared at a concentration of 20 mg/mL and treated with protease (3 h at 55 °C), papain (3 h at 37 °C), elastase (24 h at 25 °C), and α-chymotrypsin (3 h at 37 °C) as the first enzyme treatment. The hydrolysates from the first step of hydrolysis were treated with the above enzymes in different combinations and incubated for 24 h at their optimum temperatures, followed by heat inactivation at the end of each treatment. Based on 15% SDS-PAGE results, the nine best enzyme combinations were selected for further analysis. Papain + protease (PapPro, 0.0075 ± 0.004 malondialdehyde (MDA) mg/kg), α-chymotrypsin + papain (ChyPap, 0.081 ± 0.003 MDA mg/kg), and elastase + α-chymotrypsin (ElaChy, 0.083 ± 0.015 MDA mg/kg) showed strong antioxidant activity. PapPro showed the highest Fe-chelating activity (5.40 ± 0.85%) but lacked Cu-chelating activity. In conclusion, PapPro, ChyPap, and ElaChy treatments of OTF produced peptides with strong antioxidant and Fe-chelating activities but lacked Cu-chelating activity. Thus, ovotransferrin hydrolysates produced using PapPro, ChyPap, and ElaChy treatments have the potential to reduce oxidative stress in the body.