SARS-CoV-2 detection by using graphene FET arrays with a portable microfluidic measurement system

We developed graphene FET (G-FET) arrays combined with a portable microfluidic measurement system for SARS-CoV-2 detection. Multiple G-FETs modified with SARS-CoV-2 spike antibodies and those not modified were integrated onto the same chip. By calculating the difference in the FET-responses, we aime...

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Bibliographic Details
Published in:Japanese Journal of Applied Physics 2024-01, Vol.63 (1), p.16502
Main Authors: Yamamoto, Kaori, Sato, Natsuki, Sakano, Kiyoji, Kanai, Yasushi, Ushiba, Shota, Miyakawa, Naruto, Tani, Shinsuke, Kimura, Masahiko, Watanabe, Yohei, Tanaka, Hidekazu, Matsumoto, Kazuhiko
Format: Article
Language:English
Subjects:
Antibodies
Arrays
Electrical measurement
FET
Graphene
Microfluidics
Portability
SARS-CoV-2
Scanning electron microscopy
Severe acute respiratory syndrome coronavirus 2
virus
Viruses
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Summary:We developed graphene FET (G-FET) arrays combined with a portable microfluidic measurement system for SARS-CoV-2 detection. Multiple G-FETs modified with SARS-CoV-2 spike antibodies and those not modified were integrated onto the same chip. By calculating the difference in the FET-responses, we aimed to minimize noise including virus physisorption and baseline drifts. The microfluidic system was used to change ionic strengths of buffers without manual pipetting. The virus was incubated in a high ionic strength solution, followed by electrical measurements in a low ionic strength solution, leading to effective binding and electrical detection. Upon introducing the virus at a concentration of 10 8 virus ml −1 , a response of 7.9 mV was obtained. To confirm whether the response was attributed to the virus, we employed a scanning electron microscope (SEM). SEM observation indicates that the virus was much adsorbed on the antibody-modified surface compared to the non-modified surface, which agrees with the G-FET response.
ISSN:0021-4922
1347-4065
DOI:10.35848/1347-4065/ad0c45