Gene Regulation Networks Related to Neural Differentiation of hESC

With the unique property of self-renewal and developmental pluripotency, human embryonic stem cells (hESC) provide an opportunity to study molecular aspects of developmental biology. Understanding gene regulation of hESC pluripotency is a critical step toward directing hESC differentiation for regen...

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Bibliographic Details
Published in:Gene expression 2007-01, Vol.14 (1), p.23-34
Main Authors: ZHONG, JIANG F., SONG, YAHUI, DU, JING, GAMACHE, CHRISTINE, BURKE, KATHLEEN A., LUND, BRETT T., WEINER, AND LESLIE P.
Format: Article
Language:English
Subjects:
Antibodies
Apoptosis
Astrocytes
Astrocytes - cytology
Biomarkers
Cell cycle
Cell Differentiation
Cell Differentiation - genetics
Cell self-renewal
Cells, Cultured
Chromosomes
Developmental biology
Differentiation
Embryo cells
Embryo, Mammalian - cytology
Gene expression
Gene Expression Profiling
Gene Expression Regulation
Gene regulation
Gene Regulatory Networks
Humans
Insulin-like growth factor II
Microarray Analysis
Models, Genetic
Morphology
Network analysis
Neuroglia
Neuroglia - cytology
Oligonucleotide Array Sequence Analysis
Pluripotency
Pluripotent Stem Cells
Pluripotent Stem Cells - cytology
Pluripotent Stem Cells - metabolism
Pluripotent Stem Cells - physiology
Regenerative medicine
Regulation Of Gene Expression
Stem cells
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Summary:With the unique property of self-renewal and developmental pluripotency, human embryonic stem cells (hESC) provide an opportunity to study molecular aspects of developmental biology. Understanding gene regulation of hESC pluripotency is a critical step toward directing hESC differentiation for regenerative medicine. However, currently little is known about hESC gene regulation of hESC pluripotency. Applying network analysis to microarray gene expression profiling data, we compared gene expression profiles from pluripotent hESC to hESC-derived astrocytes and identified potential gene regulation networks. These gene regulation networks suggest that hECS has stringent control of cell cycle and apoptosis. Our data reveal several potential hESC differentiation biomarkers and suggest that IGF2 and A2M could play a role in hESC pluripotency by altering the availability of cytokines at the local environment of hECS. These findings underscore the importance of network analysis among differentially expressed genes, and should facilitate future study for understanding the gene regulation of hESC pluripotency.
ISSN:1052-2166
1555-3884
DOI:10.3727/000000007783991781