Histone acetylation regulates p21^WAF1 expression in human colon cancer cell lines

AIM: To investigate the effect of histone acetylation on regulation of p21^WAF1 gene expression in human colon cancer cell lines.METHODS: Two cell lines, Colo-320 and SW1116 were treated with either trichostatin or sodium butyrate.Expressions of p21^WAF1 mRNA and protein were detected by real-time R...

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Bibliographic Details
Published in:World journal of gastroenterology : WJG 2004, Vol.10 (18), p.2643-2646
Main Author: Chen, Ying-Xuan
Format: Article
Language:English
Subjects:
mRNA
p21^WAF1表达
乙酰化作用
染色质
癌细胞系统
组蛋白
结肠癌
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Summary:AIM: To investigate the effect of histone acetylation on regulation of p21^WAF1 gene expression in human colon cancer cell lines.METHODS: Two cell lines, Colo-320 and SW1116 were treated with either trichostatin or sodium butyrate.Expressions of p21^WAF1 mRNA and protein were detected by real-time RT-PCR and Western blotting, respectively.Acetylation of two regions of p21^WAF1 gene-associated histones and total cellular histones were examined by chromatin immunoprecipitation assay and Western blotting.RESULTS: Trichostatin or sodium butyrate re-activated p21^WAF1 transcription resulted in up-regulated p21^WAF1 protein level in colon cancer cell lines. Those effects were accompanied by an accumulation of acetylated histones in total cellular chromatin and p21^WAF1 gene-associated region of chromatin.CONCLUSION: Histone acetylation regulates p21^WAF1 expression in human colon cancer cell lines, Colo-320 and SW1116.
ISSN:1007-9327
2219-2840
DOI:10.3748/wjg.v10.i18.2643