A Simplified Method for the Determination of Total Iron-Binding Capacity of the Serum (TIBC)

• Published in: RADIOISOTOPES 1973/01/15, Vol.22(1), pp.12-18
• Main Authors: SAITO, Hiroshi, IGUCHI, Kenji, ODAWARA, Mitsu, NAKANE, Kiyoshi
• Format: Article
• Language: eng ; jpn
• ISSN: 0033-8303; 1884-4111
• DOI: 10.3769/radioisotopes.22.12

Serum iron was removed by Amberlite CG-400 ion exchange resin after lowering pH of the serum with citric acid. Recovering pH with sodium bicarbonate solution, the iron removed serum was incubated with radioactive ferric ammonium citrate solution and iron free transferrin was saturated with iron. Unbound iron ion in the iron solution serum mixture was removed with a resin strip made of Amberlite IRA-400, or with Amberlite CG-400. With a resin strip, ninety minutes were required for i, he removal of unbound iron ion, however, centrifugation can be omitted. With CG-400, 15 minutes were needed for the removal and then centrifugation. Clinical application of the above described method using a resin strip was per-formed with various patients and normals. The results showed a very good correlation between the TIBC by the present method and TIBC obtained by adding serum iron and unsaturated iron-binding capacity of the serum (UIBC) . Serum iron values obtained by subtracting UIBC from TIBC, and that obtained by colorimetry showed a very good correlation as well. TIBC by immunodifusion showed fairly good correlation with TIBC by the present method. The present method is simple, reproducible and can be used for the routine laboratory test.