Analysis of Oxidative DNA Damage in Patients with Colorectal Cancer

Abstract Purpose The aim of this study was to measure the levels of oxidative DNA damage in cells isolated from the colon mucosa in patients with colorectal cancer and to compare normal and neoplastic tissues and make correlations with anatomopathologic variables. Patients and Methods Thirty-three p...

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Bibliographic Details
Published in:Clinical colorectal cancer 2008-07, Vol.7 (4), p.267-272
Main Authors: Ribeiro, Marcelo L, Priolli, Denise G, Miranda, Daniel D.C, Arçari, Demétrius P, Pedrazzoli, José, Martinez, Carlos A.R
Format: Article
Language:English
Subjects:
Adenocarcinoma - genetics
Adenocarcinoma - pathology
Aged
Antioxidants
Antioxidants - therapeutic use
Colorectal Neoplasms - diagnosis
Colorectal Neoplasms - genetics
Colorectal Neoplasms - pathology
Comet Assay
DNA breakage
DNA Damage
Dukes disease
Female
Gastroenterology and Hepatology
Hematology, Oncology and Palliative Medicine
Humans
Intestinal Mucosa - pathology
Male
Middle Aged
Neoplasm Staging
Oligonucleotide Array Sequence Analysis
Oxidative Stress
Reactive Oxygen Species - metabolism
TNM staging
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Summary:Abstract Purpose The aim of this study was to measure the levels of oxidative DNA damage in cells isolated from the colon mucosa in patients with colorectal cancer and to compare normal and neoplastic tissues and make correlations with anatomopathologic variables. Patients and Methods Thirty-three patients with colorectal adenocarcinoma were studied. The oxidative DNA damage was evaluated by means of the alkaline version of the comet assay. Results For all the patients studied, it was found that the cells obtained from the neoplastic tissue presented oxidative DNA damage greater than in the cells from normal tissue. The cells isolated from the neoplastic mucosal tissue of the colon presented significantly greater mean extent of DNA strand breakage than the cells isolated from normal tissue. Additionally, the patients at earlier stages of the Dukes and TNM classifications presented higher levels of oxidative damage than those at more advanced stages. Conclusion Assessment of the levels of oxidative damage at the different stages of colorectal carcinogenesis is of great interest because it enables evaluation of the effectiveness of antioxidant substances that could be used as preventive measures against the initial oxidative aggressive action on the colonic mucosa.
ISSN:1533-0028
1938-0674
DOI:10.3816/CCC.2008.n.034