molecular phylogenetic evaluation of the Spizellomycetales

Order Spizellomycetales was delineated based on a unique suite of zoospore ultrastructural characters and currently includes five genera and 14 validly published species, all of which have a propensity for soil habitats. We generated DNA sequences from small (SSU), large (LSU) and 5.8S ribosomal sub...

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Bibliographic Details
Published in:Mycologia 2010-05, Vol.102 (3), p.596-604
Main Authors: Wakefield, William S., Powell, Martha J., Letcher, Peter M., Barr, Donald J.S., Churchill, Perry F., Longcore, Joyce E., Chen, Shu-Fen
Format: Article
Language:English
Subjects:
Agricultural soils
Animals
Biological taxonomies
chytrid
Chytridiomycota - classification
Chytridiomycota - genetics
Chytridiomycota - isolation & purification
Chytridiomycota - physiology
DNA, Fungal - analysis
DNA, Ribosomal Spacer - analysis
Forest soils
fungal anatomy
Gaertneriomyces
Genera
Glacial soils
habitats
Horticultural soils
Kochiomyces
Manure - microbiology
MOLECULAR EVOLUTION AND SYSTEMATICS
molecular systematics
new genus
Peat soils
Phylogenetics
Phylogeny
Powellomyces
ribosomal DNA
Ribosome Subunits, Small - genetics
RNA, Ribosomal, 5.8S - genetics
sequence analysis
Sequence Analysis, DNA
Soil Microbiology
species diversity
Species Specificity
Spizellomyces
Spizellomycetales
Spores, Fungal - ultrastructure
Tillage
Triparticalcar
Zoospores
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Summary:Order Spizellomycetales was delineated based on a unique suite of zoospore ultrastructural characters and currently includes five genera and 14 validly published species, all of which have a propensity for soil habitats. We generated DNA sequences from small (SSU), large (LSU) and 5.8S ribosomal subunit genes to assess the monophyly of all genera and species in this order. The 53 cultures analyzed included isolates on which all described species were based, plus other spizellomycetalean cultures. Phylogenetic placement of these chytrids was explored with maximum parsimony and maximum likelihood analyses, both of which yielded comparable topologies. Kochiomyces, Powellomyces and Triparticalcar were monophyletic, while Gaertneriomyces and Spizellomyces were polyphyletic. Isolates, distinct from described species, clustered among each of the five genera, indicating that species diversity in genera is greater than currently recognized. One isolate formed a clade that included no described species, representing a new genus. Zoospore ultrastructural features and architecture seem to be good indicators of phylogenetic relationships, but finer scrutiny of characters such as kinetosome-associated structures (KAS) is needed to understand more clearly the diversity within this order as it is revised.
ISSN:0027-5514
1557-2536
DOI:10.3852/09-120