UV radiation down-regulates Dsg-2 via Rac/NADPH oxidase-mediated generation of ROS in human lens epithelial cells

Ultraviolet radiation-induced cataract has been believed to be associated with degradation of cellular components. We report that, in cultured human lens epithelial cells, UV radiation analogous to H2O2 treatment down-regulates desmosomal protein desmoglein-2. UV radiation induces generation of reac...

Full description

Saved in:
Bibliographic Details
Published in:International journal of molecular medicine 2006-08, Vol.18 (2), p.381-387
Main Authors: Jiang, Qin, Zhou, Changlin, Healey, Sarah, Chu, Wenming, Kouttab, Nicola, Bi, Zhigang, Wan, Yinsheng
Format: Article
Language:English
Subjects:
Animals
Cell Line
Desmoglein 2 - genetics
Desmoglein 2 - metabolism
Down-Regulation
Enzyme Induction
Epithelial Cells - cytology
Epithelial Cells - metabolism
Epithelial Cells - radiation effects
ErbB Receptors - metabolism
Humans
Hydrogen Peroxide - metabolism
Lens, Crystalline - cytology
Lens, Crystalline - radiation effects
NADPH Oxidases - metabolism
Oxidants - metabolism
Phosphorylation
rac GTP-Binding Proteins - metabolism
RAC2 GTP-Binding Protein
Reactive Oxygen Species - metabolism
Ultraviolet Rays
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Ultraviolet radiation-induced cataract has been believed to be associated with degradation of cellular components. We report that, in cultured human lens epithelial cells, UV radiation analogous to H2O2 treatment down-regulates desmosomal protein desmoglein-2. UV radiation induces generation of reactive oxygen species and transiently activates epidermal growth factor receptor, which in turn induces translocation of Rac2 and NADPH oxidase activity. Collectively, our data demonstrate that UV-induced desmoglein-2 down-regulation is mediated reactive oxygen species which are generated through EGFR activation and Rac2/NADPH oxidase activation, suggesting that antioxidants may be applied for protection against UV-induced cataract.
ISSN:1107-3756
1791-244X
DOI:10.3892/ijmm.18.2.381