The Effect of Antithrombin III on Cytokine Production in Endotoxemic Rats

Antithrombin III (AT-III) supplement in severe sepsis has been thought to modulate not only coagulopathic function but also to regulate cytokine production or endothelial function. The purpose of this study is to reveal the effects of AT-III on cytokine production. [Material and methods] Wistar rats...

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Bibliographic Details
Published in:Nihon Kyukyu Igakukai Zasshi 1999/02/15, Vol.10(2), pp.91-97
Main Authors: Iba, Toshiaki, Nagakari, Kunihiko, Onodera, Kengo, Kidokoro, Akio, Ohno, Yoichi, Kaneshiro, Yoshiaki, Samejima, Tadahisa
Format: Article
Language:English
Subjects:
antithrombin III
cytokine
endotoxin
sepsis
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Summary:Antithrombin III (AT-III) supplement in severe sepsis has been thought to modulate not only coagulopathic function but also to regulate cytokine production or endothelial function. The purpose of this study is to reveal the effects of AT-III on cytokine production. [Material and methods] Wistar rats received either 250U/kg (250 group) or 500U/kg (500 group) of AT-III from a central venous catheter, while, the control group received saline alone. Thirty minutes later, 5.0mg/kg endotoxin was continuously infused for 3hrs. Blood samples were collected at 0, 3 and 6 hrs after endotoxin infusion and serum levels of rat tumor necrosis factor α (TNF), interleukin 4 and cytokine-induced neutrophil chemoattractant (CINC) were measured by ELISA. AT-III activity, fibrinogen, AST, ALT, platelet and WBC counts were examined at 6 hrs. [Results and discussion] AT-III activity was 120% in the 250 group and 170% in the 500 group. Depletion of platelet count and fibrinogen was prevented in the 500 group (p
ISSN:0915-924X
1883-3772
DOI:10.3893/jjaam.10.91