Somaclonal variation in microsperma lentil (Lens culinaris Medik)

Shoot apices of Masoor-85 seedlings (3-4 days old) induced proliferated callus masses under dark conditions, were induced to proliferate callus masses. The callus was induced under dark conditions in MS medium containing K ( 10 mg/l), GA ( 1 mg/l) with 5-10 percent lentil seed extract. The shoots we...

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Bibliographic Details
Published in:Pakistan journal of biological sciences 1999-07, Vol.2 (3), p.697-699
Main Authors: Altaf, N. (Nuclear Inst. for Agriculture and Biology, Faisalabad (Pakistan)), Iqbal, J, Ahmad, M.S
Format: Article
Language:English
Subjects:
CAL
CALLO
CALLUS
CROP YIELD
CULTIVO DE TEJIDOS
CULTURE DE TISSU
IN VITRO REGENERATION
LENS CULINARIS
REGENERACION IN VITRO
REGENERATION IN VITRO
RENDEMENT DES CULTURES
RENDIMIENTO DE CULTIVOS
SOMACLONAL VARIATION
TISSUE CULTURE
VARIACION SOMACLONAL
VARIATION SOMACLONALE
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Summary:Shoot apices of Masoor-85 seedlings (3-4 days old) induced proliferated callus masses under dark conditions, were induced to proliferate callus masses. The callus was induced under dark conditions in MS medium containing K ( 10 mg/l), GA ( 1 mg/l) with 5-10 percent lentil seed extract. The shoots were regenerated by transferring the callus culture in light. The shoots were cut and rooted to have plantlets that were finally grown in the field in winter season. These plants were Ro generation. The first selections were made from Ro and seeds were collected. These seeds were grown for R base 1 generation, whose seeds were selected for R2 generation and so on. Some selected variants were stable till R5 generation and were called somaclonal. Variants for more pods and large seed were selected.
ISSN:1028-8880
DOI:10.3923/pjbs.1999.697.699