Bioconversion of filter press cake (mud) of sugar-cane to biomass protein and its biological evaluation

Filter Press Cake (Mud) was enriched with biomass protein using Arachniotus sp. as fermentative organism. The Mud was dewaxed for increased protein production before fermentation. Proximate analysis of native and biomass was done. It was found that after fermentation ash contents decreased from 17.5...

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Bibliographic Details
Published in:Pakistan journal of biological sciences 2002-10, Vol.5 (10), p.1052-1055
Main Authors: Baig, T.T, Sheikh, M.A, Hameed, A, Jamil, A, Batool, F, Ali, S.M, Ali, S. (University of Agriculture, Faisalabad (Pakistan). Dept. of Chemistry)
Format: Article
Language:English
Subjects:
ACIDE AMINE
AMINO ACIDS
AMINOACIDOS
BIOCONVERSION
BIOMASA
BIOMASS
BIOMASSE
CANA DE AZUCAR
CANNE A SUCRE
COMPOSICION APROXIMADA
COMPOSITION GLOBALE
DECHET
DESECHOS
FERMENTACION
FERMENTATION
FILTRACION
FILTRATION
PROTEINAS
PROTEINE
PROTEINS
PROXIMATE COMPOSITION
SOUS PRODUIT DE SUCRERIE
SUBPRODUCTOS DEL AZUCAR
SUGAR BYPRODUCTS
SUGARCANE
WASTES
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Summary:Filter Press Cake (Mud) was enriched with biomass protein using Arachniotus sp. as fermentative organism. The Mud was dewaxed for increased protein production before fermentation. Proximate analysis of native and biomass was done. It was found that after fermentation ash contents decreased from 17.50 to 16.00 % and fiber was decreased from 28 to 2%. Ether extract remained the same. Prior to the production of biomass protein certain conditions like fermentation period (72 h), substrate to water ratio (6%), (NH4)2SO4(0.1 %), CaC12.2H2O (0.025%), MgSO4.7H2O (0.015%), KH2PO4 (0.1 %), Cane molasses (1.5%), and Corn Steep Liquor (2.0%) were optimized. These conditions were then applied to produce biomass protein on large scale. The biomass thus produced contained 26.25% crude protein, 13.12% true protein, 6.73% ether extract, 16% ash and 2% crude fiber. Amino acid analysis of biomass revealed the presence of 15 amino acids. The chemical score of protein was '0' and Leucine and Valine were first and second limiting amino acids respectively. The protein quality of biomass was tested in terms of digestibility that showed an average digestibility of 80.917%.
ISSN:1028-8880
DOI:10.3923/pjbs.2002.1052.1055