Regeneration of shoot from cotyledon derived callus of chickpea (Cicer arietinum L.)

Regeneration of multiple shoots via callus induction and organogenesis was achieved from cotyledon explants of chickpea (Cicer arietinum L.). Callus induction and shoot regeneration at various frequencies were observed using different concentrations and combinations of growth regulators. Highest per...

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Bibliographic Details
Published in:Pakistan journal of biological sciences 2003-08, Vol.6 (15), p.1310-1313
Main Authors: Huda, S, Siddique, N.A, Khatun, N, Rahman, M.H, Morshed, M. (Rajshahi Univ. (Bangladesh). Inst. of Biological Sciences)
Format: Article
Language:English
Subjects:
CAL
CALLO
CALLUS
CICER ARIETINUM
COTILEDONES
COTYLEDONS
COTYLÉDON
CULTIVO DE TEJIDOS
CULTURE DE TISSU
GROWING MEDIA
IN VITRO REGENERATION
ORGANOGENESIS
ORGANOGÉNESIS
ORGANOGÉNÈSE
PLANT GROWTH SUBSTANCES
REGENERACIÓN IN VITRO
RÉGÉNÉRATION IN VITRO
SUBSTANCE DE CROISSANCE VÉGÉTALE
SUBSTRAT DE CULTURE
SUBSTRATOS DE CULTIVO
SUSTANCIAS DE CRECIMIENTO VEGETAL
TISSUE CULTURE
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Summary:Regeneration of multiple shoots via callus induction and organogenesis was achieved from cotyledon explants of chickpea (Cicer arietinum L.). Callus induction and shoot regeneration at various frequencies were observed using different concentrations and combinations of growth regulators. Highest percentage (95) of callus formation was observed on MS+ 3.0 mg I-1 2,4-D+3.0 mg l-1 BAP. The maximum percentage (40) of shoot bud formation was obtained on MS medium fortified with 2.0 mg l-1 BAP and 0.5 mg l-1 NAA with number of shoots per callus was 2.50. The regenerated shoots developed highest percentage (77) roots on ® MS basal medium containing 1.0 mg l-1 IBA. Regenerated plants were successfully established in soil after acclimatization. Maximum survivability after 4 weeks of transplantation was achieved in 21 days old rooted shoots on garden soil.
ISSN:1028-8880
DOI:10.3923/pjbs.2003.1310.1313